This course consists of 40% lectures, 30% wet lab  and 30% computational work. It begins with an overview of the eDNA field, including its historical development and recent technological advances. Students will explore various applications of eDNA and learn how to analyze complex eDNA mixtures to profile their organismal components using molecular biology and DNA sequencing techniques—with an emphasis on portable and field-ready methods. The course covers all major eDNA approaches, including metagenomics, metatranscriptomics, microbiomics, and viromics, and their applications in ecosystem monitoring, species identification, anthropology, forensics, and epidemiology. In the computational component, students will gain hands-on experience using bioinformatics tools to analyze real-world datasets across these areas. Active student participation is expected and includes reading scientific literature, drafting reports, and presenting findings to the class.